Coding
AG4

Part:BBa_M10023:Design

Designed by: Dirk van Swaay   Group: Berkeley BioE140L - S09   (2009-05-07)

{˂AG4˃} Silver binding peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page] 

Wobble Odvs002F/Odvs002R to make M10023 (Bdvs002)   (67 bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5                       (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10023          {<AG4>}       (3222bp)
----
Odvs002F    Forward construction of AG4 basic part       ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC
Odvs002R    Reverse construction of AG4 basic part       ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG

Source

Source: synthetic.


References

<biblio>

  1. 1 pmid=12618805
  2. 2 Zhang Xu, Yang Peng, Yang Wantai, Chen Jinchun, The bio-inspired approach to controllable biomimetic synthesis of silver nanoparticles in organic matrix of chitosan and silver-binding peptide (NPSSLFRYLPSD), Materials Science and Engineering: C, Volume 28, Issue 2, 10 March 2008, Pages 237-242, ISSN 0928-4931, DOI: 10.1016/j.msec.2006.12.007.

</biblio>